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ISSN : 1226-4768(Print)
ISSN : 2288-1247(Online)
Food Engineering Progress Vol.18 No.1 pp.20-24

Effect of Addition of Pomegranate Powder on the Physicochemical and Sensory Qualities of Cookies

Jun Ho Lee*, Jeong In Choi
Department of Food Science & Engineering, Daegu University
Corresponding Author, Jun Ho Lee, Dept. of Food Sci. & Eng., College of Engineering, Daegu University, Gyeongsan, Gyeongbuk 712- 714, Korea Tel: 82-53-850-6535; Fax: 82-53-850-6539
December 15, 2013 January 10, 2014 January 13, 2014


Cookie samples were prepared with pomegranate powder (PP) substituted for 0%, 5%, 10%, 15%, and 20% of the wheat flour. The effect of increased levels of PP on the physicochemical properties and consumer acceptance of cookies was investigated. With the addition of PP, the pH and moisture content of both cookie dough and cookies decreased while the soluble solids content increased significantly (p < 0.05). Also lightness (L*) decreased and redness (a*) increased significantly with increasing amounts of PP in the cookies (p < 0.05). Hardness increased from 14.05 N for the control to 22.44 N for 20% PP (p < 0.05). In general, the total polyphenol content (TPC) of the cookies increased significantly with the addition of PP (p < 0.05). Acceptability ratings indicated that 10% PP content provided the optimum supplementation level for the highest consumer acceptability of the fortified cookies.


    Daegu University


    Pomegranate (Punica granatum L.) is commercially important fruits that extensively grows in may tropical and subtropical regions of the world (Tehranifar et al., 2010), but only last ten years research and commercial interests on its composition and biological properties have received the attention (Rinaldi et al., 2013). A few number of studies has been conducted in making tofu with pomegranate juice (Kim & Pakr, 2006), seockryu-pyun with pomegranate juice and concentrate (Ko et al., 2008), Korean wheat wet noodles with pomegranate cortex powder (Park et al., 2009), and jelly with pomegranate powder (PP) (Cho & Choi, 2009).

    Pomegranate has provided exceptional and unique sensory and nutritional properties, and consumption of fresh arils or juices has increased (Varela-Santos et al., 2012). The arils contains high amount of polyphenols, polysaccharides, sugars, and vitamins (Al-Maiman & Ahmad, 2002), and pomegranate juice is considered as a healthy food, due to its antioxidant properties, contributing to the prevention of cardiovascular diseases and cancers (Lansky & Newman, 2007; Sartippour et al., 2008; Basu & Penugonda, 2009). Other health beneficial effects of pomegranate juice consumption includes: reduction in blood pressure (Aviram et al., 2004), anti-HIV-1 activity (Neurath et al., 2005), inhibition of prostrate cancer in men (Pantuck et al., 2006), a reduced atherosclerosis in diabetic patients (Rock et al., 2008), and potential protection against colon cancer (Kasimsetty et al., 2010).

    Identification of ways, especially the concentration to incorporate pomegranate fruit as a health food ingredient in human diet is necessary before we can successfully use the fruit as a value-added food component. However, there is limited information on the potential for incorporation of pomegranate powder in bakery products such as cookies and its contribution to sensory characteristics and consumer acceptance of the final product. Therefore, the objectives of this research were to evaluate the impact on baking on the physicochemical properties and consumer liking of cookies incorporated with different levels of PP and to provide suitable consumer experimental data in developing new types of valueadded or functional foods.

    Materials and Methods


    Pomegranate powder (Garunara, Seoul, Korea), soft wheat flour (CJ Corp., Seoul, Korea), granulated sugar (CJ Corp., Incheon, Korea), butter (Seoul Milk Co-op., Yongin, Gyeonggi, Korea), salt (Daesang Corp., Seoul, Korea), and eggs were procured from a local market and kept at 25 °C before use.

    Dough preparation and cookie baking

    Ingredients were mixed in a Kitchen Aid mixer (model 5K5SS, Whirlpool Corp., St. Joseph, MI, USA) using a flat beater attachment as described in AACC method 10-52 (AACC, 2000) by substituting up to 20% (wt.) of PP based on the total weight of the soft wheat flour. The dough was aged for 1 h in a refrigerator at 4°C and then sheeted to a thickness of 5 mm using a rolling pin. The cookie doughs were cut with a cookie cutter to a 5 cm diameter and transferred to a lightly greased baking tray, then baked at 170°C for 12 min using a multifunctional convection oven (model GOR-704C, TongYang Magic Corp., Seoul, Korea). The baked cookies were cooled to room temperature for 1 h and packed in airtight bags before use.

    pH, moisture content, and soluble solids content of cookie and dough

    The pH of each sample (homogenized with 5 g sample + 45 mL distilled water) was measured using a pH meter (pH/Ion 510, Oakton Instruments, Vernon Hills, IL, USA). Same sample was used to determine the soluble solids contents using a digital refractometer (PR-201, Atago Co., Tokyo, Japan). The moisture content, expressed in percent wet basis (% w.b.), was measured by the gravimetric method using a laboratory convection oven (WFO-700W, Tokyo Rikakikai Co., Tokyo, Japan) at 105°C for 24 h. All measurements were repeated 5 times.

    Surface color and hardness of cookies

    Surface color at the middle of each cookie was measured with a Chromameter (model CR-200, Minolta Co., Osaka, Japan) calibrated with a white tile (Y = 94.2, x = 0.3131, and y = 0.3201). L* (lightness), a* (greenness [-] to redness [+], and b* (blueness [-] to yellowness [+]) values were recorded. All measurements were done in 5 times.

    Hardness of 20 cookies was evaluated measuring the peak breaking force (N) using the three-point break (triple beam snap) technique with a computer-controlled Advanced Universal Testing System (model LRXPlus, Lloyd Instrument Ltd., Fareham, Hampshire, UK) at room temperature. The crosshead speed was 1 mm/s and span between the two platforms was 40 mm.

    Total polyphenol content of cookies

    Total phenolics were quantified by the Folin-Denis spectrophotometric assay as previously described (Singleton et al., 1999). Briefly, 1.0 mL of filtered phenolic extract, standard or blank was mixed with 2 mL of distilled water followed by the addition of 1.0 mL of Folin-Denis reagent and 2 mL of 1 N Na2CO3. The mixture was shaken and kept in the dark for 1 h before being measured spectrophotometrically at 750 nm.

    Consumer test

    Each sample was also evaluated by 50 consumer panelists composed of university students. Five samples were presented in random order and were asked to evaluate the consumer attributes of color, flavor, taste, texture, and overall acceptability. The 9-point hedonic scale, anchored at the left as “1=extremely dislike” and at the right as “9=extremely like” was used (Peryam & Girardot, 1952). Panelists received a tray containing the samples (randomly coded using a three-digit number), a glass of water, and an evaluation sheet. Participants were advised to rinse their palates between samples and break for 30 s. Enough space was given to handle the samples and the questionnaire, and the evaluation time was not constrained.

    Statistical analysis

    The statistical analyses were performed using SAS version 9.2 (SAS Institute Inc., Cary, NC, USA). An analysis of variance (ANOVA) was performed using the general linear models (GLM) procedure to identify significant differences among the samples. Mean values were compared using Duncan's multiple range test at 5% level.

    Results and Discussion

    Selected physicochemical properties of cookie doughs

    The cookie formula used in this study contained PP. The scope of this work was to increase the functionality of cookies by adding PP, and at the same time maintaining a good sensory quality. The amount of PP was increased from 0-20% of the wheat flour and cookies with those blends are called control, PP5, PP10, PP15, and PP20.

    The effect of the PP supplementation on cookie dough characteristics, such as pH, moisture content, and soluble solids content is summarized in Table 1. pH of dough ranging from 4.33 to 6.66, decreased significantly with the addition of PP (p < 0.05). Similar significant reduction of pH was found for cookie dough prepared with shredded garlics (Kim et al., 2002), ginseng powder (Kang et al., 2009), and Saururus chinensis powder (Bae et al., 2010) as it was due to acidic nature of the powder itself.

    Moisture content of the control dough was 15.15% and gradually decreased with the higher amount of PP in the formulation (p < 0.05); however, appreciable differences were not found. On the other hand, soluble solids content increased significantly from 1.96 to 3.54 as the amount of PP increased (p < 0.05) due to the fact that PP provided more soluble solids than wheat flour in the formulation.

    Selected physicochemical properties of cookies

    Selected physicochemical properties of cookies are presented in Table 2. pH and moisture content decreased whilst soluble solids content of the cookies increased significantly (p < 0.05), as similar as in the dough. It is also noted that moisture content of the dough dropped sharply after the baking process. Lightness (L*) decreased significantly as the PP content increased in cookie (p < 0.05) as expected. Lightness of control decreased up to 32.3% when 20% PP was added in the formulation. Redness (a*), on the other hand, increased significantly as the amount of PP increased in cookie (p < 0.05). Similar changes in the lightness and redness were reported for cookies prepared with Pleurotus eryngii powder (Kim et al., 2010) and black rice bran (Joo & Choi, 2012). It is due to the natural color of PP and this suggested that the color of final product may be controlled by the amount of PP sample in the formulation.

    Hardness is one of the common indices to determine cookie quality. The hardness increased steadily from 14.05 to 22.44 N for the control and PP20, respectively (Fig. 1). Hardness of PP15 and PP20 was significantly higher than that of other samples (p < 0.05), without significant difference between them. In addition, no significant difference between the hardness of control and PP5 was found (p > 0.05). Kim and Park (2006) also reported a similar result that the hardness of tofu coagulated with pomegranate increased significantly with the higher amount of pomegranate in the formulation (p < 0.05). Hardness of cookies is closely related to its moisture content and air cell formation (Jung & Lee, 2011), and formulation (i.e., ingredients) and mixing ratios can also be factors.

    Total polyphenol content of cookies

    Total polyphenol content (TPC) of cookies as affected by the PP content is shown in Fig. 2. The TPC of control, PP5, PP10, PP15, and PP20 was 8.47, 11.85, 12.73, 15.06, and 22.49 mg/ g, respectively. It increased significantly with the addition of PP in general (p < 0.05) although no significant differences were found between samples PP5 and PP10, as well as PP10 and PP15 (p > 0.05). Ko et al. (2008) reported a similar increase in the antioxidant property in terms of DPPH radical scavenging activity when pomegranate juice or concentrate was added in seokryu-pyun. In cookie making, total polyphenol content also increased with increase in the level of Pleurotus eryngii powder (Kim et al., 2010) and black rice bran powder (Joo & Choi, 2012).

    Consumer acceptance

    The 9-point hedonic scale was used to determine which levels of PP addition to cookies were accepted by the majority of consumers. Table 3 shows the mean scores of consumer acceptance results for the five attributes including color, taste, flavor, texture, and overall acceptability. In terms of color, the control, PP5, and PP10 received the comparatively favorable mean scores ranged from 5.72 to 6.26 with no significant difference (p > 0.05), followed by PP15 sample. On the other hand, PP20 received the lowest mean score of 4.60 with respect to color among all samples tested (p < 0.05).

    The mean scores of flavor acceptability ranging from 4.68 to 5.52, and appeared the least affected by the PP content in the formulation since the control and up to 15% PP addition did not significantly influence the flavor scores (p > 0.05). The consumer acceptances on taste and texture were also significantly affected by the amount of PP incorporated in the sample (p < 0.05). It is noted that PP10 for taste, and PP15 which was not significantly different from PP10 for texture received significantly higher acceptance scores, respectively (p < 0.05).

    With respect to overall acceptability, PP10 received the highest mean score of 6.28; however, it was not significantly different from that of PP15 (p>0.05). PP20 received the lowest mean score of 3.70, significantly lower score than those of all other samples (p<0.05). PP20 was not favored for most of the consumer panelists probably due to its stronger flavor and darker color which the consumers were not used to them. Incorporation of PP into wheat flour in cookie-making showed that supplemented cookies influenced the selected physicochemical properties, and improved the sensory attributes such as flavor, taste, texture, and overall acceptability if the concentration was appropriate. PP addition at 10% of wheat flour was found to be the optimum incorporation level for the highest consumer acceptability of the cookies.



    Hardness of cookies as influenced by pomegranate powder incorporation. Means without a common letter are significantly different (p < 0.05).


    Total polyphenol content of cookies as influenced by pomegranate powder incorporation. Means without a common letter are significantly different (p < 0.05).


    Physicochemical properties of cookie doughs incorporated with pomegranate powder.

    a-eDifferent letters within the same row indicate significant difference (p < 0.05).

    Physicochemical properties of cookies incorporated with pomegranate powder.

    a-eDifferent letters within the same row indicate significant difference (p < 0.05).

    Consumer acceptance scores of cookies incorporated with pomegranate powder.

    a-eDifferent letters within the same row indicate significant difference (p < 0.05).


    1. AACC (2000) Approved methods of the AACC. Method 10-52, American Association Cereal Chemists,
    2. Al-Maiman SA , Ahmad D (2002) Changes in physical and chemical properties during pomegranate (Punica granatum L) fruit maturation , Food Chem, Vol.76; pp.437-441
    3. Aviram M , Rosenblat M , Gaitini D , Nitecki S , Hoffman A , Dornfeld L , Volkova N , Presser D , Attias J , Liker H , Hayeka T (2004) Pomegranate juice consumption for 3 years by patients with carotid artery stenosis reduces common carotid intima-media thickness, blood pressure and LDL oxidation , Clin. Nutr, Vol.23; pp.423-433
    4. Bae HJ , Lee HY , Lee JH , Lee JH (2010) Effect of Saururus chinensis powder addition on the quality of sugar snap cookies , Food Eng. Prog, Vol.14; pp.256-262
    5. Basu A , Penugonda K (2009) Pomegranate juice a hearthealthy fruit juice , Nutr. Rev, Vol.67; pp.9-56
    6. Cho Y , Choi MY (2009) Quality characteristics of jelly containing added pomegranate powder and Opuntia humifusa powder , Korean. J. Food Cookery Sci, Vol.25; pp.134-142
    7. Joo SY , Choi HY (2012) Antioxidant activity and quality characteristics of black rice bran cookies , J. Korean Soc. Food Sci. Nutr, Vol.41; pp.182-191
    8. Jung KJ , Lee SJ (2011) Quality characteristics of rice cookies prepared with sea mustard (Undaria pinnatifida Suringer) powder , J. Korean Soc. Food Sci. Nutr, Vol.40; pp.1453-1459
    9. Kang HJ , Choi HJ , Lim JK (2009) Quality characteristics of cookies with ginseng powder , J. Korean Soc. Food Sci. Nutr, Vol.38; pp.1595-1599
    10. Kasimsetty SG , Bialonska D , Reddy MK , Ma G , Khan SI , Ferreira D (2010) Colon cancer chemopreventive activities of pomegranate ellagitannins and urolithins , J. Agric. Food Chem, Vol.58; pp.2180-2187
    11. Kim HY , Jeong SJ , Heo MY , Kim KS (2002) Quality characteristics of cookies prepared with varied levels of shredded garlics , Korean. J. Food Sci. Technol, Vol.34; pp.642-646
    12. Kim JY , Park GS (2006) Quality characteristics and shelf-life of tofu coagulated by fruit juice of pomegranate , Korean. J. Food Cult, Vol.21; pp.644-652
    13. Kim YJ , Jung IK , Kwak EJ (2010) Quality characteristics and antioxidant activities of cookies added with Pleurotus eryngii powder , Korean. J. Food Sci. Technol, Vol.42; pp.183-189
    14. Ko SH , Park JH , Yoo SS (2008) Quality characteristics of seockryu-pyun added pomegranate juice and pomegranate concentrate , Korean. J. Food Cookery Sci, Vol.24; pp.722-728
    15. Lansky EP , Newman RA (2007) Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer , J. Ethnopharm, Vol.109; pp.177-206
    16. Neurath AR , Strick N , Li YY , Debnath AK (2005) Punica granatum (Pomegranate) juice provides an HIV-1 entry inhibitor and candidate topical microbicide , Ann. NY Acad. Sci, Vol.1056; pp.311-327
    17. Pantuck AJ , Leppert JT , Zomorodian N , Aronson W , Hong J , Barnard RJ , Seeram N , Liker H , Wang H , Elashoff R , Heber D , Aviram M , Ignarro L , Belldegrun A (2006) Phase II study of pomegranate juice for men with rising prostatespecific antigen following surgery or radiation for prostate cancer , Clin. Cancer Res, Vol.12; pp.4018-4026
    18. Park KT , Kim MY , Chun SS (2009) Quality characteristics of Korean wheat wet noodles with pomegranate cortex powder , Korean. J. Culin. Res, Vol.15; pp.128-136
    19. Peryam DR , Girardot NF (1952) Advanced taste test method , Food Eng, Vol.24; pp.58-61
    20. Rinaldi M , Caligiani A , Borgese R , Palla G , Barbanti D , Massini R (2013) The effect of fruit processing and enzymatic treatments on pomegranate juice composition, antioxidant activity and polyphenols content , LWT-Food Sci. Technol, Vol.53; pp.355-359
    21. Rock W , Rosenblat M , Miller-Lotan R , Levy AP , Elias M , Aviram M (2008) Consumption of wonderful variety pomegranate juice and extract by diabetic patients increases paraoxonase 1 association with high-density lipoprotein and stimulates its catalytic activities , J. Agric. Food Chem, Vol.56; pp.8704-8713
    22. Sartippour MR , Seeram NP , Rao JYU , Moro A , Harris DM , Henning SM , Firouzi A , Rettig MB , Aronson WJ , Pantuck AJ , Heber D (2008) Ellagitannin-rich pomegranate extract inhibits angiogenesis in prostate cancer in vitro and in vivo , Int. J. Oncol, Vol.32; pp.475-480
    23. Singleton VL , Orthofer R , Lamuela-Raventos RM (1999) Analysis of total phenol and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent , Method Enzym, Vol.299; pp.152-178
    24. Tehranifar A , Zarei M , Nemati Z , Esfandiyari B , Vazifeshenas MR (2010) Investigation of physico-chemical properties and antioxidant activity of twenty Iranian pomegranate (Punica granatum L) cultivars , Sci. Horti-Amsterdam, Vol.126; pp.180-185
    25. Varela-Santos E , Ochoa-Martinez A , Tabilo-Munizaga G , Reyes JE , Pérez-Won M , Briones-Labarca V , Morales-Castro J (2012) Effect of high hydrostatic pressure (HHP) processing on physicochemical properties bioactive compounds and shelf-life of pomegranate juice , Innov. Food Sci. Emerg, Vol.13; pp.13-22